Proteomics Analysis of Lungs from Somatic Cell Nuclear Transfer (SCNT) Bama Miniature Pig (Sus scrofa)

With the advent of transgenic pigs using the somatic cell nuclear transfer (SCNT), SCNT pig was thought to hold great promise in basic research, as well as in agricultural and biomedical applications, including xenotransplantation [1,2]. However, SCNT in pigs is limited by substantial problems with the current technologies, including extremely low efficiency, early embryonic and fetal mortality, postnatal mortality and abnormalities associated with SCNT [3,4]. Furthermore, many surviving cloned animals frequently have organ abnormalities including respiratory and metabolic deficiencies in their organs [5,6]. These abnormalities are likely caused by a lack of, or abnormal expression of developmentally important proteins which could affect the later development of surviving cloning animals [7,8].